Microbial DNA diagnostics
EcoGene can provide a number of microbial services:
- Species identification from pure cultures
Our standard approach consists of DNA extraction, sequence of a single gene (Fungal ITS or bacterial 16S) in both directions and best match identification with GenBank sequences.
- Metagenomic profiling
Using the 454 GS Junior, metagenomic profiles from environmental samples can be provided using amplicon sequencing approaches. We have available standard ITS and 16S primers that are tagged with unique barcodes (MID tags). We can provide the full service from receipt of environmental sample through to analysis, or we can work with PCR products and provide access to the raw data.
- Storage of cultures in the ICMP culture collection. Accessions are usually publically available, but we do offer a private use only option.
- Storage of dried fungal specimens in the PDD herbarium.
For more information on these and other EcoGene Microbial services please contact our Microbial Science Advisor: Bevan Weir